Two long-lost human relatives are still hiding in our DNA, revealing a far more tangled evolutionary past than anyone knew.
- Date:
- July 31, 2026
- Source:
- University of California, Berkeley
- Summary:
- Modern humans carry DNA from two mysterious extinct lineages in addition to Neanderthals and Denisovans. Their hidden genetic legacy shows that our evolutionary past was shaped by repeated mixing among many different human groups.
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Two previously unknown human relatives left traces of their DNA in people alive today, adding another layer to the genetic legacy already inherited from Neanderthals and Denisovans. The discovery reinforces a growing view of human evolution as a long history of encounters and interbreeding among many related hominin populations.
Earlier studies had found signs that Homo sapiens mixed with archaic humans beyond Neanderthals and Denisovans. Now, researchers at UC Berkeley have identified specific regions of the modern human genome that came from these unidentified ancestors and estimated when the interbreeding occurred.
The team made the discovery using a new method that examines hundreds of present-day human genomes and reconstructs ancient genealogical connections among their DNA sequences.
A Ghost Ancestor Shared by All Humans
One of the unknown groups, described by the researchers as a ghost ancestor, interbred with modern humans in Africa more than 50,000 years ago. The encounter took place before the most recent major movement of Homo sapiens out of Africa and into Europe and Asia.
DNA inherited from this lineage makes up approximately 1% of the modern human genome, an amount similar to the proportion inherited from Neanderthals. The ghost lineage separated from the ancestors of modern humans around 800,000 years ago, close to the time when Neanderthals and Denisovans also split from one another. However, the ghost population exchanged genes with modern humans at an earlier point than the Neanderthal and Denisovan encounters outside Africa.
"Previous publications suggested that there might be ghost ancestry -- ancestry from unknown archaic lineages in modern humans -- but they hadn't concluded whether this unknown ancestry is present only in Africans or not, and when this introgression event happened," said Berkeley graduate student Yulin Zhang, one of two first authors of the study. "We were actually able to find and map genomic locations in modern humans that are from this ghost lineage and show that this ghost ancestry is in all modern humans, not only in Africans."
DNA From a Million-Year-Old Lineage
The researchers also detected genetic material from an even more ancient population, which they call a super-archaic ancestor. This hominin descended from a lineage dating back about 1.8 million years and appears to have interbred with Denisovans in Eurasia, probably more than 200,000 years ago.
Denisovans later mixed with Homo sapiens, allowing a small portion of that super-archaic DNA to enter the genomes of modern humans.
"The super-archaic finding is particularly exciting because it reveals genetic contributions from a human lineage that lived over a million years ago, despite the absence of any sequenced DNA from that population," said Arjun Biddanda, a postdoctoral researcher at Johns Hopkins University and a co-first author of the study
Together, the findings suggest that early Homo sapiens lived alongside numerous related human groups in Africa and Eurasia during the past million years. These populations were genetically similar enough to have children together, leaving behind lasting biological traces.
"With ancient DNA from Neanderthals and Denisovans and with these new genealogical methods, we are learning that mixture among human populations has been very pervasive across time, and that this is also likely to be true at ancient time scales," said Priya Moorjani, a Berkeley associate professor of molecular and cell biology. "We often think of human evolution as a branching tree, but new genomic data and analytical methods reveal a much more interconnected history -- more like a complex web of populations connected by repeated episodes of migration and mixing."
Scientists still do not know the identities of the ghost and super-archaic populations. However, the estimated dates when they separated from other human lineages overlap with Middle Pleistocene Homo groups living in Africa around 800,000 years ago and Homo erectus populations living in Eurasia around 1.8 million years ago, respectively.
Zhang, Biddanda, Moorjani and their colleagues published their findings online July 30 in the journal Science.
Tracing Archaic DNA Without Fossils
Modern humans who left Africa roughly 50,000 years ago encountered Neanderthals and Denisovans in Eurasia and interbred with both groups. Although Neanderthals and Denisovans later disappeared, portions of their genomes survived in their modern human descendants.
Scientists uncovered that inheritance by extracting and sequencing DNA preserved in Neanderthal and Denisovan fossils. Yet the genomes of living people also contained signs of much older episodes of interbreeding.
Those signals were difficult to investigate because scientists do not have sequenced DNA from most other extinct hominins.
To overcome that limitation, Moorjani's team created a technique called TRACE (TRacking Archaic Contributions via ARG Estimation). Instead of relying on ancient remains, TRACE searches for archaic genetic regions using complete genomes from present-day people.
The researchers examined genomes from individuals around the world and reconstructed the relationships among DNA segments over many generations. This reconstruction, known as an ancestral recombination graph (ARG), produces a detailed picture of how different parts of the genome are connected through shared ancestry.
"Genealogies preserve a record of our evolutionary past," said Moorjani. "TRACE reconstructs those histories across the genome. By identifying regions whose ancestry extends unusually far back in time, we can uncover genetic contributions from extinct human populations, even in the absence of ancient DNA."
Ancient Signals Hidden in Modern Genomes
Some of the oldest genetic regions identified by TRACE matched DNA from a sequenced Neanderthal genome. Neanderthal ancestry accounts for about 1% of the human genome.
The method also successfully identified known Denisovan DNA when the team focused on genomes from Asia and Oceania, where some populations carry substantial Denisovan ancestry.
Many other ancient regions, however, matched neither Neanderthals nor Denisovans. The researchers concluded that these sections came from two separate archaic lineages that interbred with other groups at different times.
The ghost ancestry appeared in both African and non-African populations. That widespread distribution indicates that the interbreeding happened before Homo sapiens made their final major migration out of Africa and expanded around the planet.
"We discovered that about 2% of the modern human genome is from archaic hominins," Zhang said. "In the case of the ghost lineage, modern-day Africans and non-African populations both inherited similar amounts of ghost ancestry. Each individual has about 0.5 to 1% of their genome inherited from this ghost lineage."
Super-Archaic DNA Traveled Through Denisovans
The second unknown lineage became visible when researchers examined genomes from populations in Oceania, including people living across the island nations of the Pacific Ocean. These populations can carry Denisovan ancestry totaling as much as 4%.
The super-archaic genetic signal appeared within sections of Denisovan DNA. This pattern suggests that the ancient lineage first contributed DNA to Denisovans, who later passed a portion of it to modern humans.
Denisovan genomes contain an estimated 3% to 5% super-archaic ancestry. Because only some Denisovan DNA entered modern human populations, people alive today inherited only a small fraction of that much older genetic material, according to Moorjani.
"TRACE allowed us to contextualize how the ancestry segments from these previously uncharacterized hominins are distributed throughout the human genome," Biddanda said. "We found that these contributions are widespread throughout the genome, and ghost ancestry is detected even in regions previously thought to be intolerant of Neanderthal and Denisovan ancestry."
Ancient Interbreeding May Have Helped Humans Adapt
Many of the archaic DNA segments were concentrated in genomic regions connected with immune defenses and metabolism, Moorjani said.
"This pattern is not entirely surprising," she said. "Adaptation to new pathogens and food sources has been one of the strongest selective pressures in human evolution. Interbreeding with other human groups introduced new genetic variation, providing additional raw material for natural selection. Beneficial variants could then be retained and spread over many generations."
When migrating human populations entered unfamiliar environments, genes inherited from other hominins may have helped them respond to new diseases, climates, or foods. Useful variants could then become more common as they were passed from one generation to the next.
More Lost Human Lineages May Remain
Moorjani hopes that TRACE will eventually uncover weaker signals from additional unknown populations. That possibility may improve as global genome databases include a broader and more representative range of human populations.
Additional Denisovan genomes would also give researchers more material for comparison. So far, only one has been published.
Scientists may also gain clues from protein sequences recently recovered from Homo erectus fossils. Those proteins could potentially help reveal the identity of the super-archaic population.
"I think these new computational methods that allow us to reconstruct genealogical relationships are really the next frontier in this field because they are allowing us to uncover hidden episodes from our past without requiring ancient DNA," she added.
Moorjani noted that TRACE should also work with other species, "allowing us to also uncover really different patterns across the tree of life."
Other co-authors of the paper are Sarah Johnson of Berkeley's Center for Computational Biology and Colm O'Dushlaine of 54Gene, Inc., in Washington, D.C. The work was funded by the National Science Foundation.
Story Source:
Materials provided by University of California, Berkeley. Original written by Robert Sanders. Note: Content may be edited for style and length.
Journal Reference:
- Yulin Zhang, Arjun Biddanda, Sarah A. Johnson, Colm O’Dushlaine, Priya Moorjani. Recovering signatures of archaic hominin introgression using ancestral recombination graphs. Science, 2026; DOI: 10.1126/science.aef8874
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