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Genomic insights into population structure and adaptive variation of Pimelodus yuma and Pimelodus grosskopfii in the Magdalena

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Figures Abstract The biodiversity of the Magdalena–Cauca Basin, Colombia’s main fluvial system, is under severe threat from anthropogenic activities, imperiling endemic fish species such as Pimelodus yuma and Pimelodus grosskopfii. Using a population genomic approach based on single nucleotide polymorphisms (SNPs), we analyzed 64 individuals of P. grosskopfii and 57 individuals of P. yuma collecte...
**ACADEMIC MODE** **Methodology Check:** The study employs a robust genomic approach using SNPs, which provides higher resolution than previous microsatellite studies. Sampling across ~1,600 km of the Magdalena–Cauca Basin enhances geographic coverage, addressing prior limitations. However, the sample sizes (64 *P. grosskopfii*, 57 *P. yuma*) may limit statistical power for finer-scale population structure analyses. The use of RAD-seq and stringent filtering (e.g., SNP rank > 0.4, MAF > 0.01) st...